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Staining with annexin V and propidium iodide (PI) provides researchers with a way to identify different types of cell death—either necrosis or apoptosis. Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. Apoptotic cells bound with fluorochrome-labeled Annexin V can be visualized using fluorescence microscopy or flow cytometry. The reagents provided in the kit.

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Annexin V luciferase fusion proteins supplied in the assay reagent bind to PS during early apoptosis and are detected with a simple luminescence signal. The. Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V Staining Kits. Annexin V is a protein known to bind to membrane phosphatidylserine molecules to identify apoptotic cells. It is commonly used to.

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measure Annexin V binding to apoptotic cells. The authors stained control and serum starved cells with ethidium bromide and Annexin V-FITC. Binding of Annexin V. Annexin V staining is a common method for detecting apoptotic cells. Thermo Fisher Scientific offers high-quality fluorescent annexin V conjugates as. Annexin V Staining Kits. Annexin V is a protein known to bind to membrane phosphatidylserine molecules to identify apoptotic cells. It is commonly used to.